Disaccharides as Osmoprotectants: Molecular Interactions Study from Steady-state Ensemble to Femtosecond-resolved Hydration Dynamics

Small molecular osmolytes are used in nature to stabilize solvated protein structures. Disaccharides such as trehalose and sucrose appear unique in protecting biological organisms from diverse physical stresses by effectively decreasing the dynamics of water molecules when solvated. This property has led to the widespread use of disaccharides in the cosmetic, pharmaceutical, and food industries, and even to customization of disaccharides to emphasize desirable features and substitute natural disaccharides, as seen in the employment of artificial sweetener sucralose. In order to identify which molecular traits are critical to the ability of a disaccharide as a strong bioprotectant, a variety of computational and experimental techniques with a wide range of time resolution was employed. Halogenation of disaccharide may greatly alter its biopreservation ability according to spectroscopic analysis comparing sucrose and sucralose, a result consistent with other literatures. Our computational analysis revealed that numerous molecular features including dipole moment and glycodisic link properties can be affected by halogenating disaccharides, the consequences of which are important to consider in the context of biomolecular interactions.

    Item Description
    Name(s)
    Author: Cho, Inha
    Thesis advisor: Othon, Christina
    Date
    April 15, 2015
    Extent
    68 pages
    Language
    eng
    Genre
    Physical Form
    electronic
    Discipline
    Rights and Use
    In Copyright – Non-Commercial Use Permitted
    Restrictions on Use

    Access limited to Wesleyan Community only. Please contact wesscholar@wesleyan.edu for more information.

    Digital Collection
    PID
    ir:2043